Biomolecular NMR Laboratory
NMR Research Centre
Indian Institute of Science, Bangalore




  Publications
  • S. Mulleti, A. Singh, V. P. Brahmkhatri, K. Chandra, T. Raza, S. P. Mukherjee, C. S. Seelamantula, H. S. Atreya. Super-resolved Nuclear Magnetic Resonance Spectroscopy
    Sci Rep. 7:9651 (2017) [Link]

  • K. C. Rashmi KC, H. S. Atreya, M. Harsha Raj, B. P. Salimath, H. S. Aparna. A pyrrole-based natural small molecule mitigates HSP90 expression in MDA-MB-231 cells and inhibits tumor angiogenesis in mice by inactivating HSF-1.
    Cell Stress Chaperones. 22: 751-756 (2017) [Link]

  • J. Seow, R. A. Morales, C. A. MacRaild, B. Krishnarjuna, S. McGowan, T. Dingjan, G. Jaipuria, R. Rouet, K. L. Wilde, H. S. Atreya, J. S. Richards, R. F. Anders, D. Christ, N. Drinkwater, R. S. Norton. Structure and Characterisation of a Key Epitope in the Conserved C-Terminal Domain of the Malaria Vaccine Candidate MSP2.
    J. Mol. Biol. 429: 836-846 (2017) [Link]

  • F. D'Souza, S. M. Pudakalakatti, S. Uppangala, S. Honguntikar, S. R. Salian, G. Kalthur, R. Pasricha, D. Appajigowda, H. S. Atreya & S. K. Adiga. Unraveling the association between genetic integrity and metabolic activity in pre-implantation stage embryos. 
    Sci. Rep. 6:37291 (2016) [Link]

  • I. Pal, .V.Brahmkhatri, S. Bera, D. Bhattacharyya, Y. Quirishi, A. Bhunia and H. S. Atreya. Enhanced Stability and Activity of an Antimicrobial peptide in conjugation with Silver Nanoparticles.
    J. Colloid Inter Sci.  483: 385-393 (2016) [Link]

  • S. Uppangala, S. M. Pudakalakatti, F. D'Souza, S. R. Salian, G. Kalthur, P. Kumar, H. S. Atreya and S. Adiga. Influence of sperm DNA damage on human preimplantation embryo metabolism.
    Reprod. Biol. 16: 234–241 (2016) [Link]

  • S. Uppangala, F. D’Souza, S. Pudakalakatti, H. S. Atrey, K. Raval, G. Kalthur, S. K. Adiga. Laser assisted zona hatching does not lead to immediate impairment in human embryo quality and metabolism.
    Sys. Biol. Reprod. Med. 16: 396-403 (2016) [Link]

  • N. Venkatesha, Y. Qurishi, H. S. Atreya, C. Srivastava. Effect of core-shell nanoparticle geometry on the enhancement of the proton relaxivity value in a nuclear magnetic resonance experiment.
    RSC Advances 6: 64605-64610 (2016) [Link]

  • R. V. Kadumuri, J. Gullipalli, S. Subramanian, G. Jaipuria, H. S. Atreya, R. Vadrevu. Crowding interactions perturb structure and stability by destabilising the stable core of alpha-subunit of Tryptophan Synthase.
    FEBS Lett. 590: 2096-2105 (2016) [Link]

  • S. Mondal, R. Thirupathi and H. S. Atreya. Unraveling the dynamics of protein-graphene oxide interactions.
    RSC Advances 6: 52539-52548 (2016) [Link]

  • A. Dubey, Somnath Mondal, Kousik Chandra and H. S. Atreya. Rapid Identification of Amino Acid types in Proteins using phase modulated 2D HN(CACB) and 2D HN(COCACB)
    J. Magn. Reson. 267: 22-29 (2016). [Link]

  • N. Khaneja, A. Dubey A, H. S. Atreya. Ultra broadband NMR spectroscopy using multiple rotating frame technique.
    J. Magn. Reson. 265:117-128 (2016) [Link]

  • B. Krishnarjuna , S. S. Lim, S. M. Devine, C. O. Debono, R. Lam, I. R. Chandrashekaran , G. Jaipuria, H. Yagi, H. S. Atreya , M. J. Scanlon, C. A. MacRaild, P. J. Scammells, R. S. Norton. Solution NMR characterization of apical membrane antigen 1 and small molecule interactions as a basis for designing new antimalarials.
    J Mol Recognit. 2016 doi: 10.1002/jmr.2529. [Link]

  • A. Dubey, R. V. Kadumuri , G. Jaipuria, R. Vadrevu, H. S. Atreya. Rapid NMR Assignments of Proteins by Using Optimized Combinatorial Selective Unlabeling.
    Chembiochem. 17:334-40 (2016) [Link]

  • N. Venkatesha, Y. Qurishi, H S. Atreya ,C. Srivastava.  ZnO coated CoFe2O4 nanoparticles for multimodal bio-imaging
    RSC Adv. 6: 18843-18851 (2016) [Link]

  • A.Dubey, A. Rangarajan, D. Pal, H.S. Atreya. Chemical Shifts to Metabolic Pathways: Identifying Metabolic Pathways from a Single 2D NMR Spectrum.
    Anal. Chem. 87: 12197-12205 (2015). [Link]

  • Venkatesha N,  S. M Pudakalakatti,   Y. Qurishi,   H. S Atreya  and  C. Srivastava MnFe2O4-Fe3O4 core-shell nanoparticles as potential contrast agent for magnetic resonance imaging.
    RSC Advances 5: 97807-97815 (2015) [Link]

  • Varsha P Brahmkhatri, K. Chandra, A. Dubey, H.S. Atreya. An ultrastable conjugate of silver nanoparticle and protein formed through weak interactions
    Nanoscale 7:12921-12931 (2015) [Link]

  • A. Dubey, A. Rangarajan, D. Pal, H.S. Atreya.A pattern recognition based approach for identifying metabolites in NMR based metabolomics
    Anal. Chem. 87: 7148–7155 (2015) [Link]

  • C. Prasanna, A. Dubey, H.S.Atreya. Amino Acid Selective Unlabeling in Protein NMR Spectroscopy
    Methods Enzym. 565: 167-189 (2015) [Link]

  • S.S. Patil, R. Railkar, M. Swain, H.S. Atreya, R.R. Dighe, P. Kondaiah. Novel anti IGFBP2 single chain variable fragment inhibits glioma cell migration and invasion
    J Neurooncol. 123(2):225-35 (2015) [Link]

  • S. M. Pudakalakatti, A. Dubey and H. S. Atreya. Simultaneous acquisition of three NMR spectra in a single experiment for rapid resonance assignments in metabolomics.
    J. Chem. Sci. 127: 1091-1097 (2015) [Link]

  • Somnath Mondal, R. Thirupathi and H. S. Atreya. Carbon Quantum dots as a macromolecular crowder.
    RSC Adv. 2015, 5 (6), 4489 - 4492 [Link]

  • Asampille Gitanjali, Varsha P Brahmkhatri, H. S Atreya. Nanomaterial based magnetic resonance imaging of Cancer.
    J. Ind. Inst. Sci. 94, 423-454 (2014) [Link]

  • Shivanand Pudakalakatti, Kousik Chandra, Ravula Thirupathi and Hanudatta Atreya. Rapid Characterization of Molecular Diffusion by NMR.
    Chem. Eur. J. 20, 15719-15722 (2014) [Link]

  • Varsha Brahmkhatri, Chinmayi Prasanna, and Hanudatta S. Atreya. Insulin-like Growth Factor (IGF) system in Cancer: Novel Targeted Therapies
    Biomed Research International (2014; In Press) [Link]

  • Suresh, Venkata; Bonakala, Satyanarayana; Atreya, Hanudatta; Balasubramanian, Sundaram; Maji, Tapas Amide functionalized microporous organic polymer (Am-MOP) for selective CO2 sorption and catalysis
    ACS Applied Materials & Interfaces, 2014 Apr 9;6(7):4630-7 [Link]

  • Shivanand M. Pudakalakatti, A. Dubey,  Garima Jaipuria, U. Shubhashree, Satish Kumar Adiga, Detlef Moskau, Hanudatta S. Atreya A fast NMR method for resonance assignments: application to metabolomics.
    J. Biomol. NMR. 58, 165-173 (2014) [Link]
     
  • R. Thirupathi, E. Prabhakaran and H. S. Atreya Organic Fragments from Graphene Oxide: Isolation, Characterization and Solvent Effects
    J. Chem. Sci Vol. 126, 541-545 (2014) [Link]

  • S. Mondal, D. Shet, C. Prasanna, H. S. Atreya. High yield expression of proteins in E. coli for NMR studies
    Advances in Bioscience and Biotechnology 4: 751-767 (2013) [Link]

  • A. Thakur, K. Chandra, A. Dubey, P.D'Silva and H.S. Atreya. Rapid Characterization of Hydrogen Exchange in Proteins.
    Angew. Chem. Int. Ed. 52 (Issue 9): 2440-2443 (2013) [Link]

  • H.S. Atreya, B. Sathyamoorthy, G. Jaipuria, V. Beaumont, G. Varani, T. Szyperski. GFT projection NMR for efficient 1H/ 13C sugar spin system identification in nucleic acids.
    J. Biomol. NMR 54: 337-342 [Link]

  • G. Jaipuria, B. Krishnarjuna, S. Mondal, A. Dubey, and H. S. Atreya. Amino acid selective labeling and unlabeling for protein resonance assignments.
    Advances in Experimental Medicine and Biology 992: 95-116 (2012) [Link]

  • G. Jaipuria, N. P. Lobo, D. Shet and H. S. Atreya. High resolution methyl selective 13C-NMR in solution and solid state.
    J. Biomol. NMR 54: 33-42 (2012) [Link]

  • Pudakalakatti SM, Uppangala S, D'Souza F, Kalthur G, Kumar P, Adiga SK, Atreya HS. NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques: a new biomarker for assessment of embryo implantation potential.
    NMR Biomed. 26: 20-27 [Link]

  • A. Thakur, B. Chitoor, A. V. Goswami,G Pareek, H. S. Atreya, P. D’Silva. Structure and mechanistic insights into novel iron-mediated moonlighting functions of human J-protein Cochaperone, Dph4
    J. Biol. Chem 287: 13194-205 (2012) [Link]

  • M. Rout, P. Mishra, H. S. Atreya and R. V. Hosur. Reduced dimensionality 3D HNCAN for unambiguous HN, CA and N assignment in proteins
    J. Magn. Reson. 216:161-168 [Link]

  • K. Chandra, G. Jaipuria, D. Shet and H. S. Atreya. Efficient sequential assignment in proteins with reduced dimensionality 3D HN(CA) NH.
    J. Biomol. NMR 52: 115-126 (2012) [Link]

  • G. Jaipuria, A. Hayat and H. S. Atreya. New methods for NMR spectral analysis.
    J. Anal. Sci. Tech. 2 (Suppl A):A88-A93 (2011) [Link]

  • B. Krishnarjuna, G. Jaipuria, A. Thakur A, P. D’ Silva and H. S. Atreya. Amino acid selective unlabeling for sequence specific resonance assignments in proteins.
    J. Biomol. NMR 49: 39-51 (2011) [Link]

  • T. Govindaraju, M. Pandeeswar, K. Jayaramulu, G. Jaipuria and H. S. Atreya. Spontaneous self-assembly of cyclic dipeptide (Phg-Phg) into two dimensional nano and mesosheets.
    Supramol. Chem. 23: 487-492 (2011) [Link]

  • M. Swain, B. Harsha and H. S. Atreya (Book chapter). Insulin-like growth factor system in HIV/AIDS: a structure based approach for the design of new therapeutics.
    HIV and AIDS, INTECH Publishers pp 125-142 (2011) [Link]

  • H.S.Atreya. NMR methods for fast data acqusition
    Journal of the Indian Institute of Science 90(1): 87-104 (2010) [Link]

  • M. Swain, R. Tirupati, B. Krishnarjuna, E. M. Eaton, M. M. Kibbey, S. A. Rosenzweig and H. S. Atreya. Spontaneous and reversible self-assembly of a polypeptide fragment of insulin-like growth factor binding protein-2 into fluorescent nanotubular structures.
    Chem Comm 46: 216-218 (2010) (featured- inside front cover and RSC news) [Link]

  • M. Swain, M. G. Slomiany, S. A. Rosenzweig and H. S. Atreya. High-yield bacterial expression and structural characterization of recombinant human insulin-like growth factor binding protein-2.
    Arch Biochem Biophys. 501:195-200 (2010) [Link]

  • S. Rosenzweig and H. S. Atreya. Defining the pathway to insulin-like growth factor system targeting in cancer.
    Biochem Pharmacol. 80:1115-24 (2010) [Link]

  • 1.G. Jaipuria, A. Thakur, P. D’Silva and H. S. Atreya. High resolution methyl edited GFT NMR experiments for protein resonance assignments and structure determination.
    J. Biomol. NMR 48: 137-145 (2010) [Link]

  • Trent F, Atreya H.S. , Szyperski T, Rienstra C. GFT projection NMR spectroscopy of proteins in the solid state.
    J. Biomol. NMR 48: 213-223 (2010) [Link]

  • M. Swain and H. S. Atreya CSSI-PRO: CSSI-PRO: a method for secondary structure type editing, estimation and assignment in proteins using linear combination of backbone chemical shifts.
    J. Biomol. NMR. 44: 185-194 (2009) [Link]

  • G. Liu, F. Forouhar, A. Eletsky, H. S. Atreya, J. M. Aramini, R. Xiao, Y. J. Huang, M. Abashidze, J. Seetharaman, J. Liu, B. Rost B, T. Acton, G. M. Montelione, J. F. Hunt and Szyperski T. NMR and X-RAY structures of human E2-like ubiquitin-fold modifier conjugating enzyme 1 (UFC1) reveal structural and functional conservation in the metazoan UFM1-UBA5-UFC1 ubiquination pathway.
    J. Struct Funct Genomics. 10: 127-136 (2009) [Link]

  • M. Swain and H. S. Atreya A method to selectively observe a desired linear combination of chemical shifts in GFT projection NMR spectroscopy.
    Open Mag. Reson. J. 1: 96-104 (2008) [Link]

  • R. P. Barnwal, A. K. Rout, H. S. Atreya and K. V. R. Chary. Identification of C-terminal neighbours of amino acid residues without an aliphatic 13C γ as an aid to NMR assignments in proteins.
    J. Biomol. NMR. 41: 191-197(2008) [Link]

  • R. P. Barnwal, H. S. Atreya and K. V. R. Chary. Chemical shift based editing of CH3 groups in fractionally (13) C-labelled proteins using GFT (3, 2)D CT-HCCH-COSY: stereospecific assignments of CH(3) groups of Val and Leu residues.
    J. Biomol. NMR. 42: 149-154 (2008) [Link]

  • R. P. Barnwal, A. K. Rout, K. V. R. Chary and H. S. Atreya. Rapid measurement of Pseudocontact shifts in paramagnetic proteins by GFT NMR Spectroscopy.
    Open. Magn. Reson. J. 1, 16-28 (2008) [Link]

  • Q. Zhang, H. S. Atreya, D. E. Kamen, M. E. Girvin and Szyperski T. GFT projection NMR based resonance assignment of membrane proteins: application to subunit C of E.coli F(1) F (0) ATP synthase in LPPG micelles.
    J. Biomol. NMR 40: 157-163 (2008) [Link]

  • R.P. Barnwal, A.K. Rout, K.V.R. Chary and H.S. Atreya. Rapid measurement of 3JHNHα and 3JNHB in proteins.
    J. Biomol. NMR 39, 259-263 (2007) [Link]

  • H. S. Atreya, E. Garcia, Y. Shen and T. Szyperski. J-GFT NMR for precise measurement of mutually correlated spin-spin couplings.
    J. Am. Chem. Soc. 129, 680-692 (2007) [Link]

  • S. Mukherjee, D. Muralidhar, H. S. Atreya, T. Szyperski, A. Jeromin, Y. Sharma and K. V. R. Chary. 1H, 13C, and 15N chemical shift assignments of neuronal calcium sensor-1, a multi-functional calcium-binding protein.
    J. Biomol. NMR 36, 48-48 (2006) [Link]

  • S Mukherjee, H.S. Atreya, F Thomas, Y Sharma, KVR Chary. Research Paper Importance of a Single Hydrophobic Residue in the E-Helix of an EF-Hand Peptide on its Calcium Binding Affinity.
    Calcium Binding Proteins 1: 115-119 (2006) [Link]

  • T. Szyperski and H. S. Atreya. Principles and applications of GFT projection NMR spectroscopy.
    Mag. Reson. Chem. 44, S51-S60 (2006) [Link]

  • H. S. Atreya, A. Eletsky and T. Szyperski. Resonance assignment of proteins with high shift degeneracy based on 5D spectral information encoded in highly resolved G2FT NMR experiments.
    J. Am. Chem. Soc. 127, 4554-4555 (2005) [Link]

  • H. S. Atreya and T. Szyperski. Rapid NMR Data Collection.
    Methods Enzymol. 394, 78-108 (2005) [Link]

  • G. Liu, Y. Shen, H. S. Atreya, D. Parish, Y. Shao, D. K. Sukumaran, R. Xiao, A. Yee, A. Lemak, A. Bhattacharya, T. A. Acton, C. H. Arrowsmith, . T. Montelione, and T. Szyperski. NMR data collection and analysis protocol for high-throughput protein structure determination.
    Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005) [Link]

  • A. Eletski, H. S. Atreya, G. Liu and T. Szyperski. Probing structure and functional dynamics of (large) proteins with aromatic rings: L-GFT-TROSY (4,3) D HCCH NMR spectroscopy
    J. Am. Chem. Soc. 127, 14578-14579 (2005) [Link]

  • Y. Shen, H. S. Atreya, G. Liu and T. Szyperski. G-Matrix Fourier transform NOESY based protocol for high-quality protein structure determination.
    J. Am. Chem. Soc. 127, 9085-9099 (2005) [Link]

  • Y. Shen, S. Goldsmith-Fischman, H. S. Atreya, T. Acton, L. Ma, R. Xiao, B. Honig, G. T. Montelione and Thomas Szyperski. NMR structure of the 18 kDa protein CC1736 from Caulobacter crescentus identifies a member of the “START” domain superfamily and suggests residues mediating substrate specificity.
    Proteins: Struc. Func. Genet. 58, 747-750 (2005) [Link]

  • S. Mukherjee, S. M. Mustafi, H. S. Atreya and K. V. R. Chary. Measurement of 1J(Ni, Cαi), 1J(Ni, C’i-1), 2J(Ni, Cαi-1), 2J(HNi, C’i-1) and 2J(HNi, Cαi) values in 13 C/15N-labeled proteins Mag.
    Reson. Chem. 43, 326-329 (2005) [Link]

  • C. H. Penhoat, Z. Li, H. S. Atreya, S. Kim, A. Yee, R. Xiao, D. Murray, C. H. Arrowsmith and T. Szyperski. NMR solution structure of Thermotoga maritima protein TM1509 reveals a Zn-metalloprotease-like tertiary structure
    J. Struc. Func. Genomics 6, 51-62 (2005) [Link]

  • G. Liu, J. Aramini, H. S. Atreya, A. Eletski, R. Xiao, T. Acton, L. Ma, G. Montelione and T. Szyperski. GFT NMR based resonance assignment for the 21 kDa human protein CGI-126 (HSPC 155) .
    J. Biomol. NMR 32, 261-261 (2005) [Link]

  • H. S. Atreya and T. Szyperski. G-matrix Fourier Transform NMR spectroscopy for complete protein resonance assignment.
    Proc. Natl. Acad. Sci. U.S.A. 101, 9642-947 (2004) [Link]

  • Y. Shen, H. S. Atreya, R. Xiao, T. B. Acton, R. Shastry, L. Ma, G. T. Montelione, T. Szyperski. Resonance assignments for the 18 kDa protein CC1736 from Caulobacter crescentus.
    J. Biomol. NMR 29, 549-550 (2004) [Link]

  • C. H. Penhoat, H. S. Atreya, Y. Shen, G. Liu, T. B. Acton, Z. Li, D. Murray, G. T. Montelione and T. Szyperski. The NMR solution structure of the 30S ribosomal protein S27e encoded in the gene RS27_ARCFU of Archaeoglobus fulgidis reveals a novel protein fold.
    Protein Sci. 13, 1407-1416 (2004) [Link]

  • L. Yuan, H. Zeng, K. Yamato, A. R. Sanford, W. Feng, H. S. Atreya, D. K. Sukumaran, T. Szyperski and Bing Gong. Helical aromatic oligoamides: Reliable, readily predictable folding from the combination of rigidified structural motifs.
    J. Am. Chem. Soc. 126, 16528-16537 (2004) [Link]

  • S. Mukherjee, A. Sheth, S. Mustafi, H. S. Atreya and K.V.R. Chary. NMR of EF-hand calcium binding proteins.
    Proc. Indian Natl. Sci. Acad. 70(A), 615-625 (2004).

  • H. S. Atreya, S. Mukherjee, K.V. R. Chary, Y. M. Lee and C. Luchinat. Structural basis for sequential displacement of Ca2+ by Yb3+ in a protozoan EF-hand calcium binding protein.
    Protein Science 12, 412-425 (2003) [Link]

  • H. S. Atreya, K. V. R. Chary and G. Govil. Amino acid selective “unlabeling”: A new tool for structural studies of proteins in solution by NMR.
    Proc. Natl. Acad. Sci. India 73(A), 273-282 (2003).

  • H. S. Atreya and K. V. R. Chary. New chemical shift signatures of bound calcium in EF-hand proteins.
    Current Science 83, 1240-1245 (2002) [Link]

  • H. S. Atreya, K. V. R. Chary and G. Govil. Automated NMR assignments of proteins for high throughput structure determination: TATAPRO II.
    Current Science 83, 1372-1376 (2002) [Link]

  • K. V. R. Chary and H. S. Atreya. Structure determination of proteins in solution by nuclear magnetic resonance spectroscopy.
    Journal of Postgraduate Medicine 48, 83-87 (2002) [Link]

  • H. S. Atreya, S. C. Sahu, A. Bhattacharya, K. V. R. Chary and G. Govil. NMR derived solution structure of an EF-hand calcium binding protein from Entamoeba histolytica.
    Biochemistry 40, 14392-14403 (2001) [Link]

  • H. S. Atreya and K. V. R. Chary. Selective “unlabeling” of amino acids in fractionally 13C labeled proteins: An approach for stereospecific NMR assignments of CH3 groups in Val and Leu residues.
    J. Biomol. NMR 19, 267-272 (2001) [Link]

  • H. S. Atreya, S. C. Sahu, K.V.R. Chary and G. Govil. A Tracked approach for automated NMR assignments in proteins (TATAPRO).
    J. Biomol. NMR 17, 125-136 (2000) [Link]

  • H. S. Atreya and K. V. R. Chary. Amino acid selective “unlabeling” for residue- specific NMR assignments in proteins.
    Curent Science 79, 504-507 (2000) [Link]

  • S. C. Sahu, H. S. Atreya, S. Chauhan, A. Bhattacharya, K.V. R. Chary and G. Govil. Sequence specific 1H, 13C and 15N assignments of a calcium binding protein from Entamoeba histolytica.
    J. Biomol. NMR 14, 93-94 (1999) [Link]


  • Updated: May 12, 2016